Quantitative Analysis of Gallic acid and Quercetin by HPTLC and In vitro Antioxidant activity of Averrhoa carambola Linn

The preliminary phytochemical screening of ethanolic extracts Averrhoa carambola (A. carambola) leaves were done using the standard protocol. findings analysis exhibited occurrence Carbohydrates, Alkaloids, Steroids, Tannins, Vitamin C and flavonoids by Folin-Ciocalteu technique aluminum chloride colorimetric technique, respectively, whole phenolic or flavonoid amounts evaluated found 194.48±0.723 mg/g dry extract as equivalent to gallic acid 54.83±0.108 quercetin respectively. In vitro, antioxidant activity was 2,2-diphenlyl-1-picrylhydrazyl (DPPH), Hydrogen peroxide (H2O2), Nitric oxide (NO) method. Ethanolic A. showed good vitro action. Thin layer chromatography (TLC) carried out with reference biomarkers. HPTLC (High-Performance Layer Chromatography applied detect spots quantification for quercetin. Rf values 0.25 0.53 be 502.7 µg 458.3 µg/100 mg separately.